Evidence from ITS sequence data for the origin of Eupatorium godfreyanum as a derivative of E. rotundifolium/E. sessilifolium. Note the
“stutter” effect in the E.
godfreyanum sample where there
is a poly-T of 5 bp
in E. rotundifolium and of 4 bp in E. sessilifolium;
note also the bp polymorphism (orange arrow); the peak from E.
sessilifolium (G) is higher than
that from E. rotundifolium (A), which may a reflect a higher genomic contribution from E. sessilifolium to the triploid allopolyploid.
4 T’s
5 T’s
“stutter”
E. godfreyanum 2364
E. rotundifolium 2903
E.
sessilifolium 2478
Direction of sequencing – all samples