Eupatorium semiserratum complex – 3 morphologically similar species each share a 1 bp deletion in ITS-1 vs. all
other Eupatorium (see dotted red arrow in upper right, compared to E. serotinum); and the 3 species each have unique indel
features:a 3 bp deletion in ITS-1 characterizes E. linearifolium (below, black lines/arrow);
a 3 bp insertion in ITS-2 characterizes E.
semiserratum (lower right; black lines/arrow; dot highlights an additional single bp difference).